How to Autoclave Dental Instruments (UK Guide)

Smile Architect
Sep 6
2 min read

Sterilisation is one of the most critical processes in dentistry. In the UK, dental practices must meet HTM01-05

and ISO BS EN 13060 requirements to ensure instruments are properly decontaminated. This guide explains how to autoclave dental instruments step by step, the science behind the process, and the best practices every dental team should follow.

Mocom Autoclaves — Advanced dental autoclaves designed for top-class sterilization in dental practices, featuring sleek interfaces and modern design.

Why Dental Autoclaves Are Essential

Protect patients from cross-infection
Meet CQC requirements for infection prevention
Comply with HTM01-05 (UK) and ISO BS EN 13060 (European) standards
Ensure traceability of sterilisation cycles

Step-by-Step Guide to Autoclaving Dental Instruments

1.Pre-clean and Inspect Instruments

Rinse or manually clean visible debris.
Use washer-disinfectors or ultrasonic baths where available.
Check for damage or wear as only intact instruments should be autoclaved.

2. Packaging and Loading

Use autoclave-compatible pouches or wraps.
Seal instruments securely, leaving space for steam penetration.
Do not overload the autoclave chamber.

3. Selecting the Correct Cycle

Choose the cycle recommended by the manufacturer.
For dental practices in the UK, Type B autoclaves are recommended under HTM01-05 for hollow instruments and complex loads.

4. Running the Cycle

Autoclaves sterilise by using pressurised steam at 134–137°C for a minimum of 3 minutes holding time (HTM01-05 guidance).
Follow the validated cycle parameters for your device.

5. Drying and Storage

Allow the cycle to complete drying before removing pouches.
Store sterile packs in a clean, dry, and dust-free environment.
Use instruments within the recommended shelf life (check HTM01-05 guidance).

6. Practices for Dental Autoclaving

Validate your autoclave at installation and regularly thereafter.
Perform daily and weekly testing (e.g. vacuum test, Bowie-Dick test where applicable).
Keep accurate logbooks and cycle printouts for CQC inspections.
Train all staff in decontamination protocols.
Use RO or distilled water unless your autoclave (e.g. some Mocom or Stern Weber models) has a filtration system that allows tap water.

7.Compliance and Regulations in the UK

HTM01-05: Key Department of Health guidance on decontamination in dental practices.
ISO BS EN 13060: Standards for small steam sterilizers.
CQC: Practices must demonstrate safe infection control procedures during inspections.

8. Choosing the Right Dental Autoclave

Different practices have different needs. Entry-level autoclaves suit smaller clinics, while high-capacity or smart models benefit busy multi-surgery practices. Leading options include:

Mocom autoclaves – advanced features, some models allow tap water use.
Stern Weber autoclaves – reliable performance and ease of use.

Final Thoughts

Autoclaving is not just a routine , it’s a cornerstone of patient safety and professional compliance. By following HTM01-05 guidance and best practices, your dental team can ensure instruments are sterilised effectively every time.

For advice on choosing or validating an autoclave, contact Diamed Engineering, trusted suppliers and service partners for UK dental practices.